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Red light therapy is generally used as a body contouring support treatment rather than a direct fat-removal procedure

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To prepare membranes containing CTL1, 100 g of cells was thawed in 200 ml lysis buffer supplemented with protease inhibitor cocktail tablets from Thermo Fisher Scientific (1 tablet per 100 ml), Benzonase from Merck (1 mg/ml), and BioLock from Thermo Fisher Scientific (500 l/100 ml)

Flow cytometry analysis was performed using FSC/SSC gating to identify single cells and exclude debris, followed by SSC-A versus SSC-H gating to remove doublets