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Mechanistic investigations revealed OBBs dual regulatory capacity: (i) AMPK hyperphosphorylation-mediated bioenergetic enhancement through UCP1 upregulation, and (ii) inflammation resolution via inhibiting aberrant phosphorylation of IRS-1 coupled with coordinated modulation of immunometabolic signalingsuppressing proinflammatory mediators (MCP-1, CD68, Nos2, CD11c) while activating the downstream protein expression and phosphorylation (PI3K, p-Akt/Akt and p-GSK-3/GSK-3) [215]

The frozen sections were washed with PBS to wash off the embedding agent, and an appropriate amount of 200 nM Mito Tracker Red CMXRos Working Solution was added to the section samples and incubated at 37C for 30 min

Neuroscience Research 37 (3): 227-36

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