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Ascorbate has been shown to be a molecule involved in all these stages (Figure 2)

The vector for protein production, pJB-GST-HTS-HS-GFP, was created by first cloning a 7 amino acid spacer fragment containing an XhoI site into pET-15b (Novagen) using sticky ligation and BamHI/XhoI sites

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Given the metabolic plasticity of CSCs, simultaneously targeting both glycolytic and mitochondrial respiration pathways may represent a more effective strategy for tumor eradication