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The exposure media contained 50, 100 and 500 ng/mL of nicotine in CSE and the same concentrations were used for plain nicotine (EMD Millipore, Burlington, MA, USA)

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It is no surprise, therefore, that in the U.S., 82 of the 89 Pre-Market Tobacco Application authorisations for smoke-free alternatives to cigarettes have been granted to large tobacco companies

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