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Hattingen et al., 2009)

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For those who are visual learners, our friends over at the MorelliFit YouTube channel often have excellent, practical demonstrations of various lab and research techniques that can be incredibly helpful for seeing these principles in action

SF3B1 pull down assay coupled to mass spectrometry analysis 1 g of purified GST-tagged SF3B1(1463) in 48 l of kinase buffer (20 mM HEPES-KOH pH=7.9, 5 mM MgCl 2 , 2 mM DTT, 2 mM ATP, 1x phosphatase inhibitor cocktail (Sigma, P0044)) was incubated with 12 l flag-tagged CDK11 kinase (immunoprecipitated from HEK293 cells) or with 12 l of elution buffer (20 mM HEPES-KOH pH=7.9, 150 mM KCl, 1 mM DTT)

Together, Zhang and Talalay analyzed the vegetables and found that broccoli was notably high in a compound called sulforaphane, which seemed to trigger the increased production of enzymes that help cells resist toxic substances, including carcinogens