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NAD+ may take 23 minutes of gentle swirling to fully dissolve

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incubation in citrate buffer, quenching of peroxidase activity, incubation with primary antibodies for B-cell lymphoma 2 (Bcl-2) (Rabbit pAb antibody, Cat # A16776, Abclonal, Cummings Park, Woburn, USA) at dilution of 1:100 at 4 C for about 24 h

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