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Capillary electrophoresis-time of flight mass spectrometry (CE-TOFMS) was performed using an Agilent CE capillary electrophoresis system equipped with an Agilent 6210 time of flight mass spectrometer, Agilent 1100 isocratic high-performance liquid chromatography (HPLC) pump, Agilent G1603A CE-MS adapter kit, and Agilent G1607A CE-electrospray ionization (ESI)-MS sprayer kit (Agilent Technologies, Waldbronn, Germany)

By molecular simulation, we have previously shown that YMU1 acts on the catalytic pocket of TMPK, which causes the expulsion of the lid domain to give an open conformation that inhibits the catalytic process 19