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To further understand the connection between reproduction, longevity, and lipid metabolism, we selected three mutants, representing three different types of self-sterility, for detailed studies: the germline-less glp-1(e2144) , the feminized fem-3(e1996) and the masculinized mog-3(q74)

The GRADE assessment rated 36 outcomes (26%) as High, 34 (24%) as Moderate, 31 (22%) as Low, and 40 (28%) as Very Low

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We also summarize emerging biomarkers and ongoing clinical trials, and consider challenges such as inter-individual variability and the need to establish causal relationships