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Activity was monitored by the formation of a fluorescent product (coumarin) generated by a two-step coupled reaction that involved deacetylation of substrate by SIRT-1 followed by secondary release of the fluorophore

( B ) Flow cytometry of SSP4 stained HeLa cells treated with RSL3 (5 M) for 4 hours

Scientists first identified it because of its remarkable ability to stimulate tissue repair and wound healing

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