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Analysis of neutrophil gelatinase-associated lipocalin (NGAL) in mouse urine Concentrations of NGAL/Lcn-2 were determined in urine samples using the mouse lipocalin-2/NGAL DuoSet ELISA kit plus the DuoSet ELISA Ancillary Reagent Kit 2 from R&D systems (DY1857 and DY008), according to manufacturers protocol