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The cells were collected by centrifugation at 2000 g for 5 min and washed in phosphate buffered saline (8 gL 1 NaCl, 0.2 gL 1 KCl, 1.44 gL 1 Na 2 PO 4 , 0.24 g L 1 KH 2 PO 4 , at pH 7.5)

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At a concentration of 5 mg/mL (the concentration used at Good Hearts Health), 0.25 mg of semaglutide equals 5 units on an insulin syringe

Calculate the recovery by comparing the measured value with the expected amount of GSTM1 in the sample

The DA Gsta4 rat was thus designed and used to over-express Gsta4 both in primary cultures and in in vivo models in which siRNA towards Gsta4 was used as a knock-down tool