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The resulting pellet was re-suspended in minimal 50 mM HEPES pH 8.0, 200 mM NaCl, applied to a 1545% w/v sucrose gradient and subjected to ultracentrifugation at 4 C for 3 h, 105,000 g

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Serum and hepatic antioxidant activities The findings listed in Table 3 clearly illustrate that in ovo injection of Fe 3 O 4 -NPs, Cu-NPs, and ZnO-NPs during embryogenesis significantly changed the antioxidant status of the Domiaty duck strain