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An ion-trap mass spectrometry analyzer was used for tandem mass spectrometry scans in top speed mode, with dynamic exclusion settings of repeat count of 1, repeat duration of 15 s, exclusion duration of 30 s, and a cycle time of 3 s

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Clin Exp Immunol 194:153165

Differential distribution of spermidine/spermine-like immunoreactivity in neurons of the adult rat brain

This assay measures the reaction of MDA with thiobarbituric acid (TBA), which generates an MDA-TBA adduct that can be quantified colorimetrically (optical density: 532 nm)