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declares having received consulting honoraria from Amgen, AstraZeneca, Bayer, Boehringer Ingelheim, Daichii-Sankyo, esanum, Gilead, Ipsen, Eli Lilly, Novartis, Novo Nordisk, Sandoz and Sanofi

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& Richardson, D

Although metalloregulator engineering and rational or targeted mutagenesis campaigns geared toward biosensor applications have provided valuable new information on how transition metal selectivity emerges, we believe nonguided design paths such as directed evolution or more recent ligand-aware artificial intelligence tools may ultimately emerge as the new standard approach for biosensor development