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A 2023 meta-analysis found that saffron supplementation can enhance contrast sensitivity and slow vision loss progression, making it a valuable addition to modern eye formulas.58 Astaxanthin complements the formula by crossing the bloodretinal barrier and helping protect photoreceptor cells from oxidative stress.2 When combined with lutein and zeaxanthin, it has improved visual sharpness, eye fatigue, and contrast recovery after glare exposure in human studies.14 While MacuGuard provides 6mg of astaxanthin rather than the 12mg found in our top recommendations, its combination of carotenoids and saffron remains a compelling option for readers focused primarily on eye health

Clustering and UMAP visualization were conducted on the merged dataset using 10 to 30 principal components and a resolution between 0.4 and 1.2 for the shared nearest neighbor clustering algorithm (depending on the respective dataset)

The control test tube absorbance was subtracted from the test tube absorbance to exclude compounds that might impair the modified GPx-DTNB method's accuracy

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