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GLP-1RAs reduce the expression of cholesterol synthesis and bile acid synthesisassociated genes by inhibiting SREBP-2 in HepG2 cells treated with PA To investigate whether SREBP-2 inhibition mediated by GLP-1RAs affects the expression of cholesterol metabolism-related genes, HepG2 cells were transfected with SREBP-2 siRNA for 24 hours, followed by PA and GLP-1RA treatments

Interestingly, we observed that in both study groups, the changes in the abundances of the bacteria and functional pathways were not evident when comparing the baseline with the latter timepoint