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Consuming just one or two Brazil nuts can provide more than the recommended daily intake of selenium, and eating large amounts regularly may lead to adverse effects [7] , such as: Bad breath Diarrhoea Nausea Brittle hair and nails Skin rashes Joint pain Irritability Tiredness Dizziness In severe cases, extreme selenium toxicity, usually from supplements or unusually high consumption, can cause severe effects sometimes resulting in kidney failure, heart attack, or heart failure [8]

Then, 70% (v/v) Quadrol was added to a final concentration of 3% (v/v)