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The incubation mixture contained 0.9 mL of 65 mM phosphate buffer (pH7.8), 0.1 mL of 10 mM hydroxylamine hydrochloride, and 1 mL of the supernatant

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In the 2025 SURMOUNT-5 trial, adults with obesity but without diabetes received tirzepatide or semaglutide once weekly for 72 weeks