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Acknowledgements This work was funded or supported by grants of the Deutsche Forschungsgemeinschaft, project number 209933838 (SFB1052 Obesity Mechanisms: A1 to MS, B1 to MB, B3 to PK, B4 to NK, B12 to KK, C7 to JTH, Z3 to MvB) and project number 652 530364326 (to KS)

Use this formula: Concentration (mg/mL) = Vial Size (mg)BAC Water (mL) Example: a 10 mg vial reconstituted with 2 mL of BAC water yields 5 mg/mL

10.1074/jbc.M305266200 J

Step 3: Draw the Bacteriostatic Water Using your mixing syringe, pull back the plunger to draw in air equal to the amount of water you plan to use (e.g., 1 mL of air for 1 mL of water)

The door shelf experiences the most temperature fluctuation from opening and closing, so avoid storing peptides there