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After chondrocytes reached 8090% confluency, the cells were dissociated with Trypsin/EDTA (Gibco/Thermo Fisher Scientific) and passaged

doi: 10.1016/j.gendis.2019.12.008 189 TrotterEWHaganIM

Western blotting analysis Hepatocytes were lysed in RIPA buffer, and the isolated proteins were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to 0.45 m polyvinylidene difluoride membranes

However, dexrazoxane can cause bone marrow suppression (Jordan 2009), and the manufacturer of this medication reported one clinical trial in which dexrazoxane may have interfered with the clinical response to doxorubicin (Gold Standard 2012)

Curcuminoids form reactive glucuronides in vitro