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Nonetheless, in other studies, GPx1-deficient mice on a high fat diet were found to have hyperglycemia and attenuated insulin secretion due to defects in pancreatic -cell function owing to increased oxidative stress [98]

Synthesis of cDNA was performed with 1 g of total RNA from each sample using Quantiscript Reverse Transcriptase, Quantiscript RT-buffer and RT Primenr-mix of QuantiTect Reverse Transcription kit (Qiagen GmbH, Hilden, Germany) according to the manufacturers protocol

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In the Danish Prospective Diet, Cancer, and Health prospective study that followed over 57,000 men and women for 14 years, the risk of rectal cancer was found to be 42% higher in current smokers compared to nonsmokers