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Fully differentiated HepaRG cells (Lonza, catalog: NSHPRG) were plated with 90% of Williams E Medium (Thermo Fisher Scientific, catalog: A1217601) and supplemented with 10% HepaRG thawing and plating medium supplement (Lonza, catalog: MHTAP) and 25 U/mL penicillin-25 g/mL streptomycin

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A significant moment in the progression of research in this field occurred when researchers used syngeneic transplantation to place pineal glands from young mice into the thymus of older mice, resulting in a prolonged lifespan