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Cells were cultivated in EBM2 medium (Lonza, Basel, Switzerland) containing 10% FCS, 1% penicillin/streptomycin solution and 1% amphotericin B (both from Sigma-Aldrich, Taufkirchen, Germany) in dishes coated with collagen A (20 g/ml) (Biochrom, Berlin, Germany) at 37 C and 7.5% CO 2

After each addition the fluorescence of the cuvette was measured