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Although the advances obtained thank to the present projects, there is still lack of population studies for the definition of reference intervals in elite athletes populations as well as for the assessment of long-term intra-individual variation of most promising serum markers of EAAS doping

Phospho-peptide enrichment was performed using TiO 2 (Titansphere Phos-TiO Bulk 10 um, GL Science), ratio of beads to sample 10:1, mixed for 30 minutes at room temperature in 80% acetonitrile, 6% trifluoroacetic acid, 5 mM monopotassium phosphate, 20 mg/mL 2.5-dihydroxybenzoic acid

However, make sure it is not too tight

I am not seeing it in any side effect reports

The cell lysates were then boiled at 100 C for 10 minutes before use