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however, there were important limitations to consider, including small sample sizes, a lack of long-term observation, the use of a single species or strain, the lack of in-depth exploration of cellular or molecular mechanisms, failure to assess other aspects of reproductive function, a lack of validation of the clinical relevance, and a failure to consider individual differences in drug dosages

The study also confirmed that AOD9604 works by inhibiting fat synthesis in liver and fat cells, providing additional mechanistic insight into how the peptide promotes metabolic health

GAPDH (5174S, Cell Signaling Technology, 1:1000) was used as a loading control