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Skin autofluorescence (SAF) was measured using the AGE Reader (Diagnoptics, The Netherlands)

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DPP-4 cleavage of GLP-1 hGLP-1, pGLP-1, eGLP-1 and Ex-4 (30 M) were incubated with DPP-4 (3 U/L) in sodium phosphate buffer (0.1 M, pH 7.4) for 0, 30, 60, 90 and 120 min in 37 C incubator, respectively, when trifluoroacetic acid (TFA, 0.1%) was added to terminate the reactions