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The perfusion solution was then switched to solution B (composed of 67 mM NaCl, 6.7 mM KCl, 10 mM HEPES, 0.5% BSA, 4.8 mM CaCl 2 2H 2 O (all provided by Sigma-Aldrich), and 1 g l 1 collagenase P (Roche) in ddH 2 O (pH 7.6), diluted 1:2 in stop solution (composed of DPBS with Ca 2+ , Mg 2+ (Gibco), supplemented with 16.7% FBS (Merck)) and perfused at 39 C for 515 min at a rate of 25 ml min 1

The SURPASS trials showed A1c reductions of 1.24% to 2.58% depending on the dose and comparator

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Researchers continue to investigate how this multi-receptor approach differs from compounds that activate only GLP-1 or both GLP-1 and GIP receptors