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Beads were washed four times with IP wash buffer (50 mM TrisHCl (pH 7.4), 150 mM NaCl, 0.1% (vol/vol) IGEPAL CA-630), then thrice with IP wash buffer lacking IGEPAL CA-630

Reduction of oxidative stressinduced hyperpigmentation Oxidative stress from UV exposure, pollution, and inflammation can stimulate melanocytes and trigger excess melanin production, leading to dark spots and uneven tone

(6) Once a cohort of 80 animals (including 40 animals receiving NMN) had tumours that reached 60160 mm 3 in size, animals were randomly sorted into receiving either doxorubicin, cisplatin or vehicle control, for six groups (ctrl, doxorubicin, cisplatin, NMN alone, doxorubicin with NMN, cisplatin with NMN) with 10 animals in each group

7 , e2511 (2016)

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