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coli ) strain BL21, used to express Nb35, was cultured in Luria-Bertani (LB) liquid medium (10 g tryptone, 10 g NaCL and 5 g yeast extract per litre) with continuous shaking (180 rpm) or on LB agar plate (LB medium with 15 g agar per litre) at 37C

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Am J Physiol Endocrinol Metab 287:E1209E1215 Rights and permissions About this article Cite this article Faurschou, A., Knop, F.K., Thyssen, J.P