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These methods involve the use of mitochondrially-targeted compounds (e.g., oligomycin, FCCP) in conjunction with the measurement of oxygen consumption rate to assess mitochondrial respiration parameters (Seahorse XFp analyzer technology) [39, 62, 63], as well as high-resolution respirometry to evaluate mitochondrial oxidative phosphorylation (OXPHOS) capacity with complex I + II-linked substrates (Oroboros O2k-technology) [42]

Quality and handling matter just as much as choosing the correct type, since improperly stored or handled water regardless of type can compromise sterility

2.2.2 Solvent selection Following a preliminary experiment, dimethylformamide (DMF), PEG400, glycerol formal (GF), -pyrrolidone (-P) and propylene glycol (PG) were selected as potential solvents

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