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Sales are expected to continue to soar over the next several years, which will lead to an increase in calls to the poison center about side effects, dosing errors, inadvertent overdose, and unprescribed misuse by nave individuals

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The resulting supernatant was analyzed using high-performance liquid chromatography (HPLC, VWR Hitachi, Radnor, Pennsylvania, USA) coupled with mass spectrometry (3200 Q Trap, AB Sciex, Framingham, Massachusetts, USA) to quantify the target compounds

An 8 oz cup of guayusa contains roughly 100 mg of caffeine, supported by theobromine creating a state of calm alertness without the typical caffeine crash

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