doi: 10.1186/1741-7007-11-61 209 WuZ.LiuX.HuangS.LiT.ZhangX.PangJ.et al
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Flow cytometry Cell suspensions were treated with ammonium chloride-potassium (ACK) buffer (150 mmol/l NH 4 Cl, 10 mmol/l KHCO 3 and 0.1 mmol/l disodium ethylenediamine tetraacetic acid) and then stained with the following conjugated antibodies: CD3-PE (554833, BD Biosciences), CD4-fluorescein isothiocyanate (FITC) (561834, BD Biosciences), and CD8-PE (554857, BD Biosciences)
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