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No fluid retention or edema Growth hormone commonly causes water retention, leading to bloating, swelling in extremities, and a puffy appearance
However, most research connecting acupuncture and hypothyroidism consists of individual case studies, so larger-scale studies are needed to confirm this correlation
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Finally, the membranes were washed with TBST for 10 minutes 3 followed by incubation in secondary antibodies (1:10,000, Boster) for 1 hour at 37 C, visualized with ImageQuant LAS4000 system (GE Healthcare, USA)
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