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The eluted protein was dialysed overnight in the presence of His-tagged TEV protease to cleave the His 6 -MBP tag

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(8) Koustav Roy, Garima Chauhan, Punita Kumari, Meetu Wadhwa, et al (2018) Phosphorylated delta sleep inducing peptide restores spatial memory and p-CREB expression by improving sleep architecture at high altitude Life Sciences, Volume 209, 15 September 2018, Pages 282-290

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