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Step 2: Inspect and Prep Your Vials Carefully inspect both the IGF-1 LR3 vial and the Bacteriostatic Water vial

For immunofluorescence, sections were washed with PBS at room temperature twice for 10 min, followed by incubation in blocking buffer (5% fish gelatine, 0.5% Triton in PBS) at room temperature for 1 h before incubation with the primary antibodies (see Supplementary Table 4 for primary antibodies used in this study) diluted in the blocking buffer at 4 C overnight

0.5 mg Other Ingredients: Glycerin, water, highly purified phospholipids, ethanol, tocofersolan, citrus essential oils Other Ingredients: Glycerin, water, highly purified phospholipids, ethanol, tocofersolan, citrus essential oils If pregnant or breast feeding, consult your healthcare practitioner before use

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