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2.8 Determination of ROS levels The ROS were assessed via an ROS assay kit (Beyotime, China)

Slight changes in pH have been reported to interfere with amino acid functional group ionization and impair hydrogen bonding, as a result protein folding is changed, leading to denaturation and cessation of enzymatic or other activities ( Variation of pH in the environment has a direct impact on the availability of Al, Fe, Mn, Cu, and plant growth

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