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In vivo , intra-articular BoNT/A administration reduced cartilage degradation in OA rats (172)

Furthermore, we confirm that the initial glutathionylation of the glutaredoxin moiety and not the glutathione transfer to the roGFP2 moiety is rate-limiting for the oxidation of fusion constructs at physiological or low micromolar GSSG concentrations, thus allowing glutaredoxin structure-function analysis in vivo

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To further validate these findings, we replaced the AH of MmSNX1 with that of AtSNX1 and evaluated its membrane remodeling ability