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Peptides were eluted into tubes (Eppendorf) with 1 200 l and 2 100 l elution buffer (0.1% TFA in 50% aqueous MeCN) followed by a final centrifugation step (3,000 g , 3 min, r.t.)

At 3.5 months, mutant samples exhibited a significant 44% increase in DA levels along with a higher concentration of DA primary metabolites, specifically 3,4-dihydroxyphenylacetic acid (DOPAC) at 54% and homovanillic acid (HVA) at 36% (Fig

Patients should measure doses using the units shown on their own prescription rather than instructions written for another medication or person