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To aliquot safely: Work in a clean environment, ideally with minimal air movement Use sterile technique throughout Prepare labeled sterile vials or containers in advance Draw from the master vial and dispense into individual containers Refrigerate all aliquots immediately The trade-off is that aliquoting introduces more handling steps where contamination can occur

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This examination can help determine risk of EWDRpatients who have pre-existing moderate to severe NPDR are more likely to progress to PDR within one to two years after initiation of GLP-1 agonist treatment