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For alpaca immunizations, vesicles were resuspended in 20 mM HEPES pH 8, 150 mM NaCl and 250 mM sorbitol

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A subset of dedifferentiated -cells were at early stage to convert into -cell in LOsG-treated animals As a subset of FoxO1-deficient -cells adapts the -cell fate, resulting in hyperglucagonemia 22 , we determined pancreatic glucagon mRNA in LOsG-treated -cells, which were associated with low or absent FoxO1 expression

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