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In particular, Bcl-2 and Bcl-xL inhibit apoptosis by maintaining mitochondrial membrane integrity whereas Bax and Bak facilitate apoptosis by initiating the loss of outer mitochondrial integrity [21]

Different membranes for each primary antibody were used, each then incubated with a pan-H3 loading control antibody and secondary antibodies with intervening three 5-min washes with phosphate buffer saline with 0.1% Tween-20 (0.1% PBS-T)

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These results suggest that the interaction between G3BP and ASPLs UBX+ region enhances G3BP condensation, alters the material properties of the SG condensate, and stabilizes the interaction of G3BP with other SG constituents, ultimately reducing the exchange rate between SG-localized G3BP with cytosolic G3BP and promoting SG formation