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Quantitative Real-Time PCR (qRT-PCR) was used to measure mRNA expression of SASP factors (IL-1, IL-6, TNF-, TGF-) in TM3 cells, normalizing data to GAPDH
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The mobile phase consisted of a mixture of ACN and water with 0.1% TFA and was degassed by ultrasonication
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Furthermore, with average wait times for neurology specialists now exceeding a month, patients are often losing the critical window for immediate repair, allowing acute injuries to calcify into chronic Post-Concussion Syndrome (PCS)
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