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The cleaved protein was further purified by size-exclusion chromatography on a HiLoad 26/60 Superdex 200 (Amersham Biosciences) isocratically in 20 mM HEPES pH 6.8, 10% glycerol and 1 mM TCEP

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Adults exposed to secondhand smoke are at risk of several serious health problems, including: 1

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Wise says Paycheck yelled, "What do you think I am, a country hick?," and shot him