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For all in vitro assays the NAPstar sensor protein was quantified by Bradford assay 77 and the sensor protein was used at a final concentration of 240480 nM

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This precluded a placebo effect, and it produced promising results

amygdalina leaf extracts, oral administration of a single dose of 5,000 mg/kg did not produce any harmful symptoms or mortality in rats, indicating an LD 50 greater than 5,000 mg/kg (652)