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We utilized Superscript III reverse transcriptase enzyme (Invitrogen) with random hexamer primers and 3 g of RNA from each sample to produce cDNA according to the manufacturers protocol

When our natural GLP-1 response is optimal, it supports metabolism, making it easier to maintain a stable weight and avoid blood sugar spikes

References (1) Aman, F., & Masood, S

A logical extension of this framework is the consideration of another key regulatory system -the gut microbiota, which also makes a substantial contribution to diabetes pathogenesis and interacts with the islet apparatus within the framework of the gutpancreas axis

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